9/10/2023 0 Comments Unipolar neuron dendrite![]() ![]() To learn about the differential regulation of dendritic and axonal development, a genetic screen was conducted in Drosophila and the dendritic arbor reduction 1 ( dar1) mutants were isolated that display defects in dendritic but not axonal growth. These findings show that Dar1 acts downstream of Stripe and is required to set up the correct number of tendon progenitors.ĭendrites and axons are two major neuronal compartments with differences that are critical for neuronal functions. ![]() Specifically expressed in the leg tendon precursors, loss of dar1 disrupts actin-rich filopodia formation and tendon elongation. From promising candidates, the Kruppel-like factor Dar1 was identified as a critical actor of leg tendon development. This study provides for the first time a transcriptomic profile of leg tendon precursors through fluorescence-based cell sorting. A specific developmental program underlies this unique feature of tendon-like cells in the Drosophila model. During the leg disc development, cell precursors of long tendons rearrange and collectively migrate to form a tube-shaped structure. Like in vertebrate limbs, Drosophila leg muscles make connections with specific long tendons that extend through different segments. This muscle patterning requires a precise assembly of the muscle fibers with the skeleton via a specialized connective tissue, the tendon. To ensure locomotion and body stability, the active role of muscle contractions relies on a stereotyped muscle pattern set in place during development. ![]() Transcriptomic and Genetic Analyses Identify the Kruppel-Like Factor Dar1 as a New Regulator of Tube-Shaped Long Tendon Development. Laurichesse, Q., Moucaud, B., Laddada, L., Renaud, Y., Jagla, K. These data suggest that Dar1 is a functional homolog of the mammalian KLF4. Consistent with these findings, Dar1 was found to be downregulated in the wounded midguts, allowing increased ISC proliferation to promote intestinal repair. Evidence is provided that Dar1 has a function upstream of the JAK/STAT signaling pathway, suggesting Dar1 can regulate ISC proliferation through different mechanisms. This experiment revealed that Dar1 negatively regulated the expression of several critical cell cycle genes. In order to explore the molecular mechanism of the Dar1 functions, the gene expression profiles of the Dar1 knockdown and Dar1 OE midguts was compared, using the deep RNA sequencing (RNA-Seq) technique. On the other hand, overexpression (OE) of Dar1 inhibited ISC division and blocked the formation of ISC lineages. Suppression of Dar1-activated ISC proliferation as a consequence, the ISCs and the young differentiated cells were increased. In an RNAi-based screening experiment, dendritic arbor reduction 1 (Dar1), which is a KLF member in Drosophila, inhibited the proliferation of intestinal stem cells (ISCs). The kruppel-like factors (KLFs) are a family of transcription factor proteins that regulate a wide range of biological processes. The Kruppel-like factor Dar1 restricts the proliferation of Drosophila intestinal stem cells. These data suggest that Dar1 is a functional homolog of the mammalian KLF4 (Wu, 2018). Consistent with these findings, this study discovered Dar1 was down-regulated in the wounded midguts, allowing increased ISCs proliferation to promote intestinal repair. ![]() In order to explore the molecular mechanism of the Dar1 functions, the gene expression profiles of the Dar1 knockdown and Dar1 overexpression midguts were compared, using the deep RNA sequencing (RNA-Seq) technique. On the other hand, overexpression of Dar1 inhibited ISC division and blocked the formation of ISC lineages. Suppression of Dar1 activated ISC proliferation as a consequence, the ISCs and the young differentiated cells were increased. In an RNAi-based screening experiment, this study identified Dar1, which is a KLF member in Drosophila, inhibited the proliferation of intestinal stem cells (ISCs). The kruppel like factors (KLFs) are a family of transcription factor proteins that regulate a wide range of biological processes. The kruppel like factor Dar1 restricts the proliferation of Drosophila intestinal stem cells. Keywords - dendritic arborization, regulation of cytoskeleton Dendritic arbor reduction 1: Biological Overview ![]()
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